High Throughput Sequencing

DNA sequencing is an indispensable tool that has assisted in the significant acceleration of biological research and discovery. The center's high throughput sequencing facility offers exciting capabilities including SNP mapping, RNA expression measurements, ChIP-seq and DNA Methylation analyses at a genomewide scale. Such capabilities enable researchers to study the regulation of thousands of genes in a single experiment, providing unprecedented insights into the molecular biology of a cell.

This facility provides the UCLA stem cell research community with access to the “next generation” DNA sequencing technology including: 

Library preparation: 
One custom Perkin-Elmer Janus G3 liquid handler. This device has been programmed to execute several protocols for DNA-seq (whole genome, ChIP) and RNA-seq library preparation and new protocols can be automated if requested. Requests should be submitted to Marco Morselli, Ph.D., at mmorselli@ucla.edu.  

Quality control:
One Agilent TapeStation 4200 for automated electrophoresis of 1 to 96 samples. The use of this platform is strongly recommended for all the quality control steps of next generation sequencing experiments.

Training is required prior to using either of the above intruments. Contact Marco Morselli, Ph.D., at mmorselli@ucla.edu to schedule a training.

Sequencer:
One NovaSeq 6000 sequencing instrument which generates a large amount of DNA sequences with high accuracy in a short time. 

Core Pricing: 

Service Description Read Number UCLA Rate External Rate
NovaSeq SP 1x100bp 400 million $1,709.76 $2,359.47
NovaSeq SP 2x50bp 400 million $1,709.76 $2,359.47
NovaSeq SP 2x150bp 400 million $2,490.64 $3,437.09
NovaSeq SP 2x250bp 400 million $3,295.92 $4,548.37
NovaSeq S1 1x100bp 800 million $2,612.65 $3,605.46
NovaSeq S1 2x50bp 800 million $2,612.65 $3,605.46
NovaSeq S1 2x100bp 800 million $3,295.92 $4,548.37
NovaSeq S1  2x150bp 800 million $3,832.78 $5,289.23
NovaSeq S2 1x100bp 2 billion $6,093.41 $8,408.91
NovaSeq S2  2X50p 2 billion $6,093.41 $8,408.91
NovaSeq S2 2X100bp 2 billion $7,826.67 $10,800.80
NovaSeq S2 2x150bp 2 billion $8,910.50 $12,296.49
NovaSeq S4 2x100bp 3 billion $5,113.90 $7,057.18
NovaSeq S4 2x150bp 3 billion $6,643.07 $9,167.44