High Throughput Sequencing

About

The High-Throughput Sequencing Core provides the UCLA research community with access to next-generation DNA sequencing technology. 

The technology in this Core offers capabilities including SNP mapping, RNA expression measurements, ChIP-seq and DNA methylation analyses at a genome-wide scale. 

Such capabilities enable researchers to study the regulation of thousands of genes in a single experiment, providing unprecedented insights into the molecular biology of a cell.

The Core is located in the Terasaki Life Sciences Building, room 3110.

Training & Submissions

Training:
Training is required prior to using the Perkin-Elmer Janus G3 liquid handler or the Agilent TapeStation 4200. Contact Giorgia Del Vecchio, Ph.D., at giodelv@g.ucla.edu to schedule a training and access to the scheduling site.

Submissions:
Sequencing requests are submitted through the High-Throughput Sequencing Core request site. 

Equipment

• Perkin Elmer Janus G3 liquid handler
  • A library preparation device programmed to execute several protocols for DNA-seq (whole genome, ChIP) and RNA-seq library preparation. 
• Agilent TapeStation 4200
  • A quality control system used for automated electrophoresis of 1 to 96 samples. It is strongly recommended that this platform be used for all quality control steps of next generation sequencing experiments. 
• NovaSeq 6000
  • Next-generation sequencing (NGS) system for RNA-seq, whole genome, whole exome, and targeted DNA sequencing. Generates a large amount of sequences with high accuracy in a short time. 
• iSeq 100
  • A flexible sequencing system with a wide range of applications that is ideal for small genome sequencing, targeted and amplicon sequencing, and viral and microbial sequencing.

Pricing:

Service Description	Read Number	UCLA Rate	External Rate
iSeq 100 2x150bp	4 million	$629.79	$718.63
NovaSeq SP 1x100bp	325-400 million	$2,091.65	$2,465.30
NovaSeq SP 2x50bp	325-400 million	$2,091.65	$2,465.30
NovaSeq SP 2x100bp	325-400 million	$2,553.00	$2,930.10
NovaSeq SP 2x150bp	325-400 million	$2,765.55	$3,169.50
NovaSeq SP 2x250bp	325-400 million	$3,392.30	$3,775.65
NovaSeq S1 1x100bp	650-800 million	$3,019.65	$3,403.25
NovaSeq S1 2x50bp	650-800 million	$3,019.65	$3,403.25
NovaSeq S1 2x100bp	650-800 million	$3,713.95	$4,100.25
NovaSeq S1  2x150bp	650-800 million	$4,069.50	$4,483.75
NovaSeq S2 1x100bp	1.65-2.05 billion	$5,159.35	$5,628.50
NovaSeq S2  2X50bp	1.65-2.05 billion	$5,159.35	$5,628.50
NovaSeq S2 2X100bp	1.65-2.05 billion	$6,172.30	$6,753.25
NovaSeq S2 2x150bp	1.65-2.05 billion	$6,655.35	$7,280.75
NovaSeq S4 2x100bp	2-2.5 billion	$4,793.55	$5,221.45
NovaSeq S4 2x150bp	2-2.5 billion	$5,356.20	$6,014.15
XP loading for SP, S1 and S2	N/A	$247.20	$346.70
XP loading for S4	N/A	$246.85	$345.10

*Read number based on Illumina specs

FAQs

1. How much does sequencing cost?

Please see the pricing section of this page.

2. How do I make the library pool? 

Libraries can be quality controlled by DNA gel, TapeStation, or BioAnylzer. To make a library pool for sequencing, please use Qubit BR assay to measure the library concentration, figure out the molarity of the library (using online tools such as: http://www.molbiol.ru/eng/scripts/01_07.html), dilute all the libraries that you want to pool to the same concentration (10nM is preferred, but we can accept libraries as low as 1nM) and then pool them by equal volume. The volume of the final 10nM pool should be at least 10ul (more volume is needed if the concentration is lower than 10nM). Please only submit the final pool, not individual libraries, for sequencing.

3. Who can I contact with billing questions?

Please contact Charlie Stough at CStough@mednet.ucla.edu for all billing inquiries. 

4. Are you open to external customers? 

Yes, please see our pricing section of this page to see external pricing. For more information about the process of sending samples to the Core, please contact Suhua Feng at sfeng@mcdb.ucla.edu.