High Throughput Sequencing

scientist operating an instrument in the sequencing core

About

The High-Throughput Sequencing Core provides the UCLA research community with access to next-generation DNA sequencing technology. 

The technology in this Core offers capabilities including SNP mapping, RNA expression measurements, ChIP-seq and DNA methylation analyses at a genome-wide scale. 

Such capabilities enable researchers to study the regulation of thousands of genes in a single experiment, providing unprecedented insights into the molecular biology of a cell.

The Core is located in the Terasaki Life Sciences Building, room 3110.

Training & Submissions

Training:
Training is required prior to using the Perkin-Elmer Janus G3 liquid handler or the Agilent TapeStation 4200. Contact Giorgia Del Vecchio, Ph.D., at giodelv@g.ucla.edu to schedule a training and access to the scheduling site.

Submissions:
Sequencing requests are submitted through the High-Throughput Sequencing Core request site

Equipment

  • Perkin Elmer Janus G3 liquid handler
    • A library preparation device programmed to execute several protocols for DNA-seq (whole genome, ChIP) and RNA-seq library preparation. 
  • Agilent TapeStation 4200
    • A quality control system used for automated electrophoresis of 1 to 96 samples. It is strongly recommended that this platform be used for all quality control steps of next generation sequencing experiments. 
  • NovaSeq 6000
    • Next-generation sequencing (NGS) system for RNA-seq, whole genome, whole exome, and targeted DNA sequencing. Generates a large amount of sequences with high accuracy in a short time. 
  • iSeq 100
    • A flexible sequencing system with a wide range of applications that is ideal for small genome sequencing, targeted and amplicon sequencing, and viral and microbial sequencing.

Pricing:

*Read number based on Illumina specs

Service Description

Read Number

Internal Rate

External Rate -
Non-Profits

External Rate -
Companies

iSeq 100 2x150bp

4 million

$716

$806

$838

NovaSeq 6000 SP 1x100bp  

325-400 million

$1,693

$1,885

$2,091

NovaSeq 6000 SP 2x50bp

325-400 million

$1,693

$1,885

$2,091

NovaSeq 6000 SP 2x100bp

325-400 million

$2,179

$2,403

$2,643

NovaSeq 6000 SP 2x150bp

325-400 million

$2,406

$2,662

$2,935

NovaSeq 6000 SP 2x250bp

325-400 million

$3,282

$3,606

$3,953

NovaSeq 6000 S1 1x100bp

650-800 million

$2,669

$2,861

$3,067

NovaSeq 6000 S1 2x50bp

650-800 million

$2,669

$2,861

$3,067

NovaSeq 6000 S1 2x100bp

650-800 million

$3,405

$3,629

$3,868

NovaSeq 6000 S1 2x150bp

650-800 million

$3,719

$3,975

$4,248

NovaSeq 6000 S2 1x100bp

1.65-2.05 billion

$4,765

$4,973

$5,196

NovaSeq 6000 S2 2x50bp

1.65-2.05 billion

$4,765

$4,973

$5,196

NovaSeq 6000 S2 2x100bp

1.65-2.05 billion

$5,922

$6,183

$6,462

NovaSeq 6000 S2 2x150bp

1.65-2.05 billion

$6,407

$6,721

$7,056

NovaSeq 6000 S4 2x100bp

2-2.5 billion

$4,333

$4,551

$4,785

NovaSeq 6000 S4 2x150bp

2-2.5 billion

$4,817

$5,057

$5,313

Novaseq 6000 XP loading

N/A

$196

$272

$299

FAQs

  1. How much does sequencing cost?

Please see the pricing section of this page.

  1. How do I make the library pool? 

Libraries can be quality controlled by DNA gel, TapeStation, or BioAnylzer. To make a library pool for sequencing, please use Qubit BR assay to measure the library concentration, figure out the molarity of the library (using online tools such as: http://www.molbiol.ru/eng/scripts/01_07.html), dilute all the libraries that you want to pool to the same concentration (10nM is preferred, but we can accept libraries as low as 1nM) and then pool them by equal volume. The volume of the final 10nM pool should be at least 10ul (more volume is needed if the concentration is lower than 10nM). Please only submit the final pool, not individual libraries, for sequencing.

  1. Who can I contact with billing questions?

Please contact Charlie Stough at CStough@mednet.ucla.edu for all billing inquiries. 

  1. Are you open to external customers? 

Yes, please see our pricing section of this page to see external pricing. For more information about the process of sending samples to the Core, please contact Suhua Feng at sfeng@mcdb.ucla.edu.