Flow Cytometry Core


photo credit: Flow Cytometry Data Reader and Visualization Example
rob@mathworks.com

The Flow Cytometry Core provides the UCLA research community with flow cytometry instrumentation and expertise in cell analysis and cell sorting services. The instruments use Fluoresence Activated Cell Sorting (FACS) technology to count and examine cells and DNA and provide simultaneous analysis of the physical and chemical characteristics of up to thousands of particles per second.

In order to regenerate, stem cells are usually surrounded by a support system of other cells. The Core enables our faculty to identify the stem cells they wish to study and distinguish them from the other cells in the dish or tissue. The cutting-edge instruments provide BSCRC faculty with the ability to analyze cell populations as well as isolate cells with special characteristics and characterize biochemical, genomic, and epigenetic properties. The Core is located in the Terasaki Life Sciences Building, room 3045.

Instrumentation and Fees:

Sorters (operator run only)        Usage Fees:     
  • BD FACSARIA
 $102/hr

 

Analyzers:  Usage Fees: Training Fees:
  • BD LSRII
  • BD Celesta
  • BD Fortessa     

Independent Analysis: $36.51/hr

Operator Assisted Analysis: $102/hr      

Intital Training Session: $102 (3 hours)     

Supplemental Training: $50/hr

Access and Training Requirements:
Each lab member utilizing sorting and analysis services is required to review the BSCRC FCC User Policies and submit a BSCRC FCC Agreement via email signed by both the user and their PI acknowledging the privileges and responsibilities. To gain UCLA ID card access to the Core, users are required to visit the BSCRC administrative office in 477 BSRB  during office hours (Monday and Thursday 3:30-4:30PM).

Prior to scheduling hands-on analysis training with a Core operator, users are required to complete a training request form in PPMS and complete the following online courses:

Upload certificates of completiont to PPMS with your training request.

Scheduling:
Sorting and Analysis scheduling is done through PPMS. Users are required to create a user account. For assisted analysis, contact a Flow Cytometry Operator directly to schedule an appointment. Appointments for all services can be booked no more than two months in advance. Large sort appointments are subject to director approval.